TUBA6 [p Ser165] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23260
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Chicken, Xenopus
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for TUBA6 [p Ser165] Antibody
Immunogen
Phospho-TUBA6 (Ser-165) synthetic peptide (coupled to KLH) corresponds to amino acid residues around serine 165 of human TUBA6. This sequence is well conserved in most alpha-tubulin isoforms from most eukaryotic species, but is not conserved in beta-Tubulin. [UniProt# Q9BQE3]
Modification
p Ser165
Specificity
This antibody was cross-adsorbed to unphosphorylated TUBA6 (a.a. 160-169) peptide before affinity purification using TUBA6 (Ser-165) peptide (without carrier). The antibody detects a 50 kDa protein corresponding to the molecular mass of a-Tubulin on SDS-PAGE immunoblots of recombinant a1-tubulin phosphorylated with PKC alpha. This reactivity is not observed after lambda phosphatase treatment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for TUBA6 [p Ser165] Antibody
![TUBA6 [p Ser165] Antibody](https://resources.bio-techne.com/images/products/nbp3-23260_rabbit-tuba6-p-ser165-pab-18920231735521.jpg "Western Blot: TUBA6 [p Ser165] Antibody [NBP3-23260] -")
Western Blot: TUBA6 [p Ser165] Antibody [NBP3-23260] -
Western Blot: TUBA6 [p Ser165] Antibody [NBP3-23260] - Western blot analysis of 250 ng/lane of a1-Tubulin unphosphorylated (lanes 1, 3, & 5) or phosphorylated at Ser-165 with PKC alpha (lanes 2, 4, & 6). The blots were probed with anti-TUBA6 (a.a. 160-169) (NBP3-23259; lanes 1 & 2), anti-TUBA6 (Ser-165) (NBP3-23260; lanes 3 & 4), and anti-alpha Tubulin (C-terminus) (NBP3-23258; lanes 5 & 6).Applications for TUBA6 [p Ser165] Antibody
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: TUBA6
Long Name
Tubulin alpha-1C chain
Alternate Names
Alpha-tubulin 6, TUBA1C
Gene Symbol
TUBA1C
Additional TUBA6 Products
Product Documents for TUBA6 [p Ser165] Antibody
Product Specific Notices for TUBA6 [p Ser165] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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