TUBGCP2 Antibody (GCP2-01) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21793
![TUBGCP2 Antibody (GCP2-01) - BSA Free Western Blot: TUBGCP2 Antibody (GCP2-01) - BSA Free [NBP2-21793]](https://resources.bio-techne.com/images/products/nbp2-21793_mouse-monoclonal-tubgcp2-antibody-gcp2-01-131202416381110.jpg "Western Blot: TUBGCP2 Antibody (GCP2-01) - BSA Free [NBP2-21793]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # GCP2-01
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
GST-fusion protein containing amino acids 2-194 of mouse GCP2.
Reactivity Notes
Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.
Specificity
The mouse monoclonal antibody GCP2-01 recognizes gamma-tubulin complex component 2 (GCP2), a 95-103 kDa protein. It recognizes also phosphorylated GCP2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TUBGCP2 Antibody (GCP2-01) - BSA Free
Western Blot: TUBGCP2 Antibody (GCP2-01) - BSA Free [NBP2-21793]
Analysis of human gamma-tubulin complex component 2 (GCP2) using mouse monoclonal antibody GCP2-01 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-GCP2 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for GCP2 at approximately 95 kDa.Applications for TUBGCP2 Antibody (GCP2-01) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:300
Immunohistochemistry
1:50-1:300
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:500-1:2000
Application Notes
Western Blot: can be used, but not optimal
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: TUBGCP2
Alternate Names
Gamma-ring complex protein 103 kDa, gamma-tubulin complex component 2, gamma-tubulin complex protein 2, GCP-2, GCP2hGrip103, h103p, hGCP2, hSpc97, MGC138162, SPBC97, Spc97p, Spindle pole body protein Spc97 homolog, tubulin, gamma complex associated protein 2
Entrez Gene IDs
10844 (Human)
Gene Symbol
TUBGCP2
Additional TUBGCP2 Products
Product Documents for TUBGCP2 Antibody (GCP2-01) - BSA Free
Product Specific Notices for TUBGCP2 Antibody (GCP2-01) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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