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TULA/STS-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-57842

Novus Biologicals, part of Bio-Techne
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NB100-57842

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence QQCSTPDGKYYRPD corresponding to internal region according to NP_061834.1, NP_001001895.1.

Epitope

QQCSTPDGKYYRPD

Specificity

This antibody is expected to recognize both reported isoforms (NP_061834.1 and NP_001001895.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TULA/STS-2 Antibody

Immunohistochemistry-Paraffin: TULA/STS-2 Antibody [NB100-57842]

Immunohistochemistry-Paraffin: TULA/STS-2 Antibody [NB100-57842]

Immunohistochemistry-Paraffin: TULA/STS-2 Antibody [NB100-57842] - IHC analysis of TULA/STS-2 in paraffin-embedded Human Spleen using NB100-57842 at 2.5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for TULA/STS-2 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Peptide ELISA

Detection limit 1:64000
Application Notes
WB: Preliminary experiments gave an approx. 48 kDa band in lysates of B-cell line Daudi after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 74.1 kDa band according to NP_061834.1 and 69.8 kDa band according to NP_001001895.1. The 48 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human spleen shows cytoplasm staining of T-cells eccentrically gathered around an artery.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TULA/STS-2

UBASH3A, ubiquitin associated and SH3 domain containing, A, interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors, EGFR and PDGFRB, on the cell surface (referenced from Swiss Prot).

Alternate Names

Cbl-interacting protein 4, gene similar to UBA containing SH3 domain10CLIP4STS-2T-cell ubiquitin ligand protein, STS2, Suppressor of T-cell receptor signaling 2, T-cell ubiquitin ligand, TULAubiquitin-associated and SH3 domain-containing protein A, ubiquitin associated and SH3 domain containing A

Entrez Gene IDs

53347 (Human)

Gene Symbol

UBASH3A

Additional TULA/STS-2 Products

Product Documents for TULA/STS-2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TULA/STS-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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