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TWEAK R/TNFRSF12 Antibody (1G4T3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16621

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-16621-20ul
NBP3-16621-100ul

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1G4T3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-129 of human TWEAK R/TNFRSF12 (Q9NP84). MDCASCRARPHSDFCLGCAAAPPAPFRLLWPILGGALSLTFVLGLLSGFLVWRRCRRREKFTTPIEETGGEGCPAVALIQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TWEAK R/TNFRSF12 Antibody (1G4T3)

Western Blot: TWEAK R/TNFRSF12 Antibody (1G4T3) [NBP3-16621]

Western Blot: TWEAK R/TNFRSF12 Antibody (1G4T3) [NBP3-16621]

Western Blot: TWEAK R/TNFRSF12 Antibody (1G4T3) [NBP3-16621] - Analysis of extracts of various cell lines, using TWEAK R/TNFRSF12 Rabbit mAb (NBP3-16621) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunocytochemistry/ Immunofluorescence: TWEAK R/TNFRSF12 Antibody (1G4T3) [NBP3-16621]

Immunocytochemistry/ Immunofluorescence: TWEAK R/TNFRSF12 Antibody (1G4T3) [NBP3-16621]

Immunocytochemistry/Immunofluorescence: TWEAK R/TNFRSF12 Antibody (1G4T3) [NBP3-16621] - Immunofluorescence analysis of NIH-3T3 cells using TWEAK R/TNFRSF12 Rabbit mAb (NBP3-16621) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for TWEAK R/TNFRSF12 Antibody (1G4T3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TWEAK R/TNFRSF12

TWEAK (otherwise known as TNFRSF12A, Tumor necrosis factor receptor superfamily member 12A), is a member of the TNF family and was first described as a weak inducer of apoptosis in some cell types. It is thought to promote angiogenesis and the proliferation of endothelial cells as well as modulating cellular adhesion to matrix proteins. Recently, a receptor for TWEAK was isolated by expression cloning from a HUVEC cell cDNA library.

Long Name

TNF-like and Weak Inducer of Apoptosis Receptor

Alternate Names

CD266, Fn14, TNFRSF12, TNFRSF12A, TWEAKR

Gene Symbol

TNFRSF12A

Additional TWEAK R/TNFRSF12 Products

Product Documents for TWEAK R/TNFRSF12 Antibody (1G4T3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TWEAK R/TNFRSF12 Antibody (1G4T3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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