TWEAK R/TNFRSF12 Antibody (7H8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26280
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7H8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human TWEAK R/TNFRSF12 [UniProt Q9NP84]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TWEAK R/TNFRSF12 Antibody (7H8)
Immunohistochemistry: TWEAK R/TNFRSF12 Antibody (7H8) [NBP3-26280] -
Immunohistochemistry: TWEAK R/TNFRSF12 Antibody (7H8) [NBP3-26280] - Image of TWEAK R/TNFRSF12 Antibody (7H8) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: TWEAK R/TNFRSF12 Antibody (7H8) [NBP3-26280] -
Western Blot: TWEAK R/TNFRSF12 Antibody (7H8) [NBP3-26280] - Positive Western Blot detected in: U251 whole cell lysate, MCF-7 whole cell lysate, U87 whole cell lysate.All lanes: TWEAK R/TNFRSF12 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 14, 11 kD
Observed band size: 14 kDa
Applications for TWEAK R/TNFRSF12 Antibody (7H8)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: TWEAK R/TNFRSF12
Long Name
TNF-like and Weak Inducer of Apoptosis Receptor
Alternate Names
CD266, Fn14, TNFRSF12, TNFRSF12A, TWEAKR
Gene Symbol
TNFRSF12A
Additional TWEAK R/TNFRSF12 Products
Product Documents for TWEAK R/TNFRSF12 Antibody (7H8)
Product Specific Notices for TWEAK R/TNFRSF12 Antibody (7H8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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