TWEAK/TNFSF12 Antibody (MTW-1) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-09046
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Mouse
Applications
Block/Neutralize, Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rat IgG1 kappa Clone # MTW-1
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was raised by immunising SD rats with CD8-mTWEAK, a fusion of the human CD8alpha extracellular region to murine TWEAK.
Specificity
This antibody is specific for murine TWEAK. It has been shown not to react with TNF- alpha , FasL, and TRAIL.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1 kappa
Applications for TWEAK/TNFSF12 Antibody (MTW-1) - Azide and BSA Free
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
The specificity of this antibody has been confirmed in flow cytometric analysis, where it reacts specifically with mTWEAK-transfected L5178Y cells, and not with parental L5178Y cells or cells transfected with related TNF family members (Nakayama et al, 2003). Additionally, this antibody has been used to assess TWEAK cell surface expression in flow cytometric analysis (Nakayama et al, 2003). This antibody has been shown to block CD8-mTWEAK cytotoxicity against L5178Y cells over-expressing murine Fn14, the TWEAK receptor (Nakayama et al, 2003). When administered to collagen-induced arthritis (CIA) mice, this blocking antibody has been shown to have anti-inflammatory and antiangiogenic effects, with associated improvements to both the clinical and histological manifestations of CIA (Kamata et al, 2006). Additionally, administration of this antibody to denervated mice attenuates denervation-induced skeletal muscle loss (Mittal et al, 2010). Finally, administration of this antibody to CD163-/- mice subject to hind limb ischaemia inhibits the increased perfusion resulting from CD163 deficiency (Akahori et al, 2015).
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TWEAK/TNFSF12
Long Name
TNF-like and Weak Inducer of Apoptosis
Alternate Names
TNFSF12
Gene Symbol
TNFSF12
Additional TWEAK/TNFSF12 Products
Product Documents for TWEAK/TNFSF12 Antibody (MTW-1) - Azide and BSA Free
Product Specific Notices for TWEAK/TNFSF12 Antibody (MTW-1) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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