Twist-1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB120-49254
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1 mg/ml
Product Specifications
Immunogen
The immunogen is a synthetic peptide directed towards the C terminal region of human Twist-1. Peptide Sequence DKLSKIQTLKLAARYIDFLYQVLQSDELDSKMASCSYVAHERLSYAFSVW. The peptide sequence for this immunogen was taken from within the described region.
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 26825317).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for Twist-1 Antibody
![Western Blot: Twist-1 Antibody [NB120-49254]](https://resources.bio-techne.com/images/products/Twist-1-Antibody-Western-Blot-NB120-49254-img0007.jpg "Western Blot: Twist-1 Antibody [NB120-49254]")
Western Blot: Twist-1 Antibody [NB120-49254]
Western Blot: Twist-1 Antibody [NB120-49254] - Sample Tissue: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 2.5ug/mL, Peptide Concentration: 2.0ug/mL, Lysate Quantity: 25ug/lane, Gel Concentration: 12%![Immunohistochemistry-Paraffin: Twist-1 Antibody [NB120-49254]](https://resources.bio-techne.com/images/products/Twist-1-Antibody-Immunohistochemistry-Paraffin-NB120-49254-img0002.jpg "Immunohistochemistry-Paraffin: Twist-1 Antibody [NB120-49254]")
Immunohistochemistry-Paraffin: Twist-1 Antibody [NB120-49254]
Immunohistochemistry-Paraffin: Twist-1 Antibody [NB120-49254] - Human Uterus Tissue, 4-8ug/ml.![Western Blot: Twist-1 Antibody [NB120-49254]](https://resources.bio-techne.com/images/products/Twist-1-Antibody-Western-Blot-NB120-49254-img0001.jpg "Western Blot: Twist-1 Antibody [NB120-49254]")
Western Blot: Twist-1 Antibody [NB120-49254]
Western Blot: Twist-1 Antibody [NB120-49254] - Jurkat cell lysate, Antibody Titration: 2.5ug/mlApplications for Twist-1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Twist-1
Long Name
Twist Related Protein 1 [Drosophila]
Alternate Names
ACS3, BPES3, H-twist, Twist1
Entrez Gene IDs
7291 (Human)
Gene Symbol
TWIST1
Additional Twist-1 Products
Product Documents for Twist-1 Antibody
Product Specific Notices for Twist-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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