TWISTNB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89454

Novus Biologicals, part of Bio-Techne
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NBP1-89454
NBP1-89454-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TWISTNB Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DADDTPMEESALQNTNNANGIWEEEPKKKKKKKKHQEVQDQDPVFQGSDSSGYQSDHKKKKKKRKHSEEAEFTPPLKCSP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TWISTNB Antibody

Western Blot: TWISTNB Antibody [NBP1-89454]

Western Blot: TWISTNB Antibody [NBP1-89454]

Western Blot: TWISTNB Antibody [NBP1-89454] - Analysis in control (vector only transfected HEK293T lysate) and TWISTNB over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: TWISTNB Antibody [NBP1-89454]

Immunocytochemistry/ Immunofluorescence: TWISTNB Antibody [NBP1-89454]

Immunocytochemistry/Immunofluorescence: TWISTNB Antibody [NBP1-89454] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus, nucleoli fibrillar center & microtubules.
Immunohistochemistry-Paraffin: TWISTNB Antibody [NBP1-89454]

Immunohistochemistry-Paraffin: TWISTNB Antibody [NBP1-89454]

Immunohistochemistry-Paraffin: TWISTNB Antibody [NBP1-89454] - Staining of human cerebellum shows cytoplasmic and nucleolar positivity in Purkinje cells.
View 1 more images

Applications for TWISTNB Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TWISTNB

RPA43 is a component of the RNA polymerase I complex which synthesizes ribosomal RNA precursors. RPA43 is also known as TWISTNB (twist neighbor protein), a gene identified within a genomic region (7p21) that is deleted in patients with Saethre-Chotzen syndrome and specific learning disabilities.

Alternate Names

DNA-directed RNA polymerase I subunit RPA43, TWIST neighbor, Twist neighbor protein

Gene Symbol

TWISTNB

Additional TWISTNB Products

Product Documents for TWISTNB Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TWISTNB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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