TXNDC5 Antibody (2E7/7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22414
![Immunocytochemistry/ Immunofluorescence: TXNDC5 Antibody (2E7/7) [NBP2-22414]](https://resources.bio-techne.com/images/products/TXNDC5-Antibody-2E7-7-Immunocytochemistry-Immunofluorescence-NBP2-22414-img0002.jpg "Immunocytochemistry/ Immunofluorescence: TXNDC5 Antibody (2E7/7) [NBP2-22414]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 2E7/7
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the EndoPDI protein
Localization
Endoplasmic Reticulum , Endoplasmic Reticulum Lumen
Specificity
Detects approx 48kDa.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for TXNDC5 Antibody (2E7/7)
Immunocytochemistry/ Immunofluorescence: TXNDC5 Antibody (2E7/7) [NBP2-22414]
Immunocytochemistry/Immunofluorescence: TXNDC5 Antibody (2E7/7) [NBP2-22414] - Tissue: Cervical Cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-EndoPDI Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.Applications for TXNDC5 Antibody (2E7/7)
Application
Recommended Usage
Western Blot
1:200
Application Notes
1 ug/ml of EndoPDI Antibody was sufficient for detection of EndoPDI in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary Antibody.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TXNDC5
Long Name
Thioredoxin Domain Containing 5
Alternate Names
EndoPDI, ERp46, TLP46
Gene Symbol
TXNDC5
UniProt
Additional TXNDC5 Products
Product Documents for TXNDC5 Antibody (2E7/7)
Product Specific Notices for TXNDC5 Antibody (2E7/7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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