U2AF2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-57251

Novus Biologicals, part of Bio-Techne
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NBP1-57251
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all U2AF2 Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to U2AF2 (U2 small nuclear RNA auxiliary factor 2) The peptide sequence was selected from the N terminal of U2AF2. Peptide sequence EFERQLNENKQERDKENRHRKRSHSRSRSRDRKRRSRSRDRRNRDQRSAS. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for U2AF2 Antibody

Western Blot: U2AF2 Antibody [NBP1-57251]

Western Blot: U2AF2 Antibody [NBP1-57251]

Western Blot: U2AF2 Antibody [NBP1-57251] - Sample Tissue: Human Jurkat Antibody Dilution: 1.0 ug/ml
Western Blot: U2AF2 Antibody [NBP1-57251]

Western Blot: U2AF2 Antibody [NBP1-57251]

Western Blot: U2AF2 Antibody [NBP1-57251] - Reccomended Titration: 1.25 ug/ml Positive Control: Jurkat cell lysate U2AF2 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells

Applications for U2AF2 Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: U2AF2

U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2AF2 is the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly.U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.

Alternate Names

hU2AF(65), hU2AF65, splicing factor U2AF 65 kD subunit, splicing factor U2AF 65 kDa subunit, U2 (RNU2) small nuclear RNA auxiliary factor 2, U2 auxiliary factor 65 kDa subunit, U2 small nuclear ribonucleoprotein auxiliary factor (65kD), U2 small nuclear RNA auxiliary factor 2, U2AF65U2 snRNP auxiliary factor large subunit

Gene Symbol

U2AF2

UniProt

Q96HC5 (Human)

Additional U2AF2 Products

Product Documents for U2AF2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for U2AF2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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