U2AF35 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19120

Novus Biologicals, part of Bio-Techne
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NBP1-19120
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all U2AF35 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 140 and 190 of human U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit using the numbering given in entry NP_006749.1 (GeneID 7307).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for U2AF35 Antibody

Western Blot: U2AF35 Antibody [NBP1-19120]

Western Blot: U2AF35 Antibody [NBP1-19120]

Western Blot: U2AF35 Antibody [NBP1-19120] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. U2AF35 was also immunoprecipitated by rabbit anti-U2AF35 antibody NBP1-19121.
Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-19120]

Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-19120]

Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-19120] - Section of human lung carcinoma (left) and mouse CT26 colon carcinoma (right).

Applications for U2AF35 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: U2AF35

This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Alternate Names

FP793, human U2 snRNP auxiliary factor small subunit10U2AF35DKFZp313J1712, RN, RNU2AF1,35-KD subunit, U2 auxiliary factor 35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 small nuclear RNA auxillary factor 1, U2 snRNP auxiliary factor small subunit

Entrez Gene IDs

7307 (Human); 108121 (Mouse)

Gene Symbol

U2AF1

UniProt

NP_006749.1 (Human)

Additional U2AF35 Products

Product Documents for U2AF35 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for U2AF35 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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