U2AF35 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92553

Novus Biologicals, part of Bio-Techne
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NBP1-92553
NBP1-92553-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all U2AF35 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MHLKPISRELRRELYGRRRKKHRSRS

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24579871)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for U2AF35 Antibody

Western Blot: U2AF35 Antibody [NBP1-92553]

Western Blot: U2AF35 Antibody [NBP1-92553]

Western Blot: U2AF35 Antibody [NBP1-92553] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunocytochemistry/ Immunofluorescence: U2AF35 Antibody [NBP1-92553]

Immunocytochemistry/ Immunofluorescence: U2AF35 Antibody [NBP1-92553]

Immunocytochemistry/Immunofluorescence: U2AF35 Antibody [NBP1-92553] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-92553]

Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-92553]

Immunohistochemistry-Paraffin: U2AF35 Antibody [NBP1-92553] - Staining of human hippocampus shows moderate nuclear positivity in neuronal cells.

Applications for U2AF35 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: U2AF35

U2AF35 belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Alternate Names

FP793, human U2 snRNP auxiliary factor small subunit10U2AF35DKFZp313J1712, RN, RNU2AF1,35-KD subunit, U2 auxiliary factor 35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 small nuclear RNA auxillary factor 1, U2 snRNP auxiliary factor small subunit

Gene Symbol

U2AF1

Additional U2AF35 Products

Product Documents for U2AF35 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for U2AF35 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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