Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2C5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all UAP56 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human BAT1(NP_542165) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

48.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UAP56 Antibody (OTI2C5) - Azide and BSA Free

Western Blot: UAP56 Antibody (OTI2C5)Azide and BSA Free [NBP2-74727]

Western Blot: UAP56 Antibody (OTI2C5)Azide and BSA Free [NBP2-74727]

Western Blot: UAP56 Antibody (OTI2C5) - Azide and BSA Free [NBP2-74727] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UAP56 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UAP56.
Immunohistochemistry: UAP56 Antibody (OTI2C5) - Azide and BSA Free [NBP2-74727]

Immunohistochemistry: UAP56 Antibody (OTI2C5) - Azide and BSA Free [NBP2-74727]

Immunohistochemistry: UAP56 Antibody (OTI2C5) - Azide and BSA Free [NBP2-74727] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-UAP56 mouse monoclonal antibody.
Immunohistochemistry: UAP56 Antibody (OTI2C5) - Azide and BSA Free [NBP2-74727]

Immunohistochemistry: UAP56 Antibody (OTI2C5) - Azide and BSA Free [NBP2-74727]

Immunohistochemistry: UAP56 Antibody (OTI2C5) - Azide and BSA Free [NBP2-74727] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-UAP56 mouse monoclonal antibody.
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Applications for UAP56 Antibody (OTI2C5) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UAP56

UAP56 encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and also plays an important role in mRNA export from the nucleus to the cytoplasm. A cluster of genes, BAT1-BAT5, is localized in the vicinity of the genes for TNF alpha and TNF beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternatively spliced transcript variants encoding the same protein have been described.

Alternate Names

56 kDa U2AF65-associated protein, ATP-dependent RNA helicase p47, DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B, DEAD box protein UAP56, EC 3.6.4.13, HLA-B associated transcript 1, HLA-B-associated transcript 1 protein, spliceosome RNA helicase BAT1, UAP56D6S81EBAT1nuclear RNA helicase (DEAD family)

Gene Symbol

DDX39B

Additional UAP56 Products

Product Documents for UAP56 Antibody (OTI2C5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UAP56 Antibody (OTI2C5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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