UbcH5b/UBE2D2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81769

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UbcH5b/UBE2D2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LTDLQRDPPAQCSAGPVGDDLFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTKIYHPNINSNGSICLDILRS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UbcH5b/UBE2D2 Antibody

Western Blot: UbcH5b/UBE2D2 Antibody [NBP1-81769]

Western Blot: UbcH5b/UBE2D2 Antibody [NBP1-81769]

Western Blot: UbcH5b/UBE2D2 Antibody [NBP1-81769] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: UbcH5b/UBE2D2 Antibody [NBP1-81769]

Immunocytochemistry/ Immunofluorescence: UbcH5b/UBE2D2 Antibody [NBP1-81769]

Immunocytochemistry/Immunofluorescence: UbcH5b/UBE2D2 Antibody [NBP1-81769] - Staining of human cell line U-251 MG shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: UbcH5b/UBE2D2 Antibody [NBP1-81769]

Immunohistochemistry-Paraffin: UbcH5b/UBE2D2 Antibody [NBP1-81769]

Immunohistochemistry-Paraffin: UbcH5b/UBE2D2 Antibody [NBP1-81769] - Staining of human breast shows cytoplasmic and nuclear positivity in glandular cells.
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Applications for UbcH5b/UBE2D2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UbcH5b/UBE2D2

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

Alternate Names

PUBC1, UBC5B, UBE2D2

Gene Symbol

UBE2D2

Additional UbcH5b/UBE2D2 Products

Product Documents for UbcH5b/UBE2D2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UbcH5b/UBE2D2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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