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UbcH5b/UBE2D2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20783

Novus Biologicals, part of Bio-Techne
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NBP2-20783

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Porcine (98%), Rat (100%), Xenopus (92%), Zebrafish (98%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Full length human UbcH5b/UBE2D2 Recombinant protein.

Reactivity Notes

Xenopus laevis (92%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UbcH5b/UBE2D2 Antibody

Western Blot: UbcH5b/UBE2D2 Antibody [NBP2-20783]

Western Blot: UbcH5b/UBE2D2 Antibody [NBP2-20783]

Western Blot: UbcH5b/UBE2D2 Antibody [NBP2-20783] - Sample (30 ug of whole cell lysate) A: NT2D1 B: U87-MG 15% SDS PAGE gel, diluted at 1:1000.

Applications for UbcH5b/UBE2D2 Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UbcH5b/UBE2D2

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq]

Alternate Names

PUBC1, UBC5B, UBE2D2

Gene Symbol

UBE2D2

UniProt

Additional UbcH5b/UBE2D2 Products

Product Documents for UbcH5b/UBE2D2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UbcH5b/UBE2D2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.