UbcH8/Ube2L6 Antibody (2F12-1F4)

Novus Biologicals, part of Bio-Techne | Catalog # H00009246-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2F12-1F4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UbcH8 Antibodies »

Product Specifications

Immunogen

UBE2L6 (AAH32491, 1 a.a. ~ 153 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MMASMRVVKELEDLQKKPPPYLRNLSSDDANVLVWHALLLPDQPPYHLKAFNLRISFPPEYPFKPPMIKFTTKIYHPNVDENGQICLPIISSENWKPCTKTCQVLEALNVLVNRPNIREPLRMDLADLLTQNPELFRKNAEEFTLRFGVDRPS

Reactivity Notes

Human. Other species not tested.

Specificity

UBE2L6 - ubiquitin-conjugating enzyme E2L 6

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for UbcH8/Ube2L6 Antibody (2F12-1F4)

Western Blot: UbcH8/Ube2L6 Antibody (2F12-1F4) [H00009246-M01]

Western Blot: UbcH8/Ube2L6 Antibody (2F12-1F4) [H00009246-M01]

Western Blot: UbcH8/Ube2L6 Antibody (2F12-1F4) [H00009246-M01] - Analysis of UBE2L6 expression in transfected 293T cell line by UBE2L6 monoclonal antibody (M01), clone 2F12-1F4.Lane 1: UBE2L6 transfected lysate(17.595 KDa).Lane 2: Non-transfected lysate.
Western Blot: UbcH8/Ube2L6 Antibody (2F12-1F4) [H00009246-M01]

Western Blot: UbcH8/Ube2L6 Antibody (2F12-1F4) [H00009246-M01]

Western Blot: UbcH8/Ube2L6 Antibody (2F12-1F4) [H00009246-M01] - analysis of UBE2L6 expression in transfected 293T cell line by UBE2L6 monoclonal antibody (M01), clone 2F12-1F4.
Sandwich ELISA: UbcH8/Ube2L6 Antibody (2F12-1F4) [H00009246-M01] - Detection limit for recombinant GST tagged UBE2L6 is 3 ng/ml as a capture antibody.

Applications for UbcH8/Ube2L6 Antibody (2F12-1F4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UbcH8

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is highly similar in primary structure to the enzyme encoded by UBE2L3 gene. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Alternate Names

RIG-B, UBE2L6

Entrez Gene IDs

9246 (Human)

Gene Symbol

UBE2L6

OMIM

603890 (Human)

UniProt

AAH32491 (Human)

Additional UbcH8 Products

Product Documents for UbcH8/Ube2L6 Antibody (2F12-1F4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UbcH8/Ube2L6 Antibody (2F12-1F4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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