UBE2J1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78065
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This affinity purified UBE2J1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near the amino terminus of the human UBE2J1 protein. (Uniprot: Q9Y385)
Reactivity Notes
A BLAST analysis was used to suggest cross-reactivity with UBE2J1 protein from human, mouse and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Localization
Endoplasmic reticulum
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This affinity purified antibody is directed against human UBE2J1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for UBE2J1 Antibody
Western Blot: UBE2J1 Antibody [NBP1-78065]
Western Blot: UBE2J1 Antibody [NBP1-78065] - Western blot using affinity purified UBE2J1 antibody shows detection of UBE2J1 in 293 cells over-expressing Myc-UBE2J1 (Lane 2). Lane 1 contains lysate from mock-transfected 293 cells.
N/A: UBE2J1 Antibody [NBP1-78065]
UBE2J1 Antibody
Western blot using affinity purified anti-Ube2j1 antibody shows detection of Ube2j1 in 293 cells over-expressing Myc-Ube2j1 (Lane 2). Lane 1 contains lysate from mock-transfected 293 cells. Personal Communication, A. Weissman & T. Shang, CCR-NCI, Frederick, MDApplications for UBE2J1 Antibody
Application
Recommended Usage
ELISA
1:1000-1:5000
Immunoprecipitation
1-2 ug
Western Blot
1:200-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 35-40 kDa in size corresponding to Ube2j1 protein by western blotting in the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: UBE2J1
Long Name
Ubiquitin-conjugating Enzyme E2J 1
Alternate Names
CGI-76, HSPC153, NCUBE1, UBC6e
Entrez Gene IDs
51465 (Human)
Gene Symbol
UBE2J1
UniProt
Additional UBE2J1 Products
Product Documents for UBE2J1 Antibody
Product Specific Notices for UBE2J1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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