UBE2J2/UBC6 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06347
![Western Blot: UBE2J2/UBC6 Antibody [NBP3-06347]](https://resources.bio-techne.com/images/products/UBE2J2-UBC6-Antibody-Western-Blot-NBP3-06347-img0001.jpg "Western Blot: UBE2J2/UBC6 Antibody [NBP3-06347]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for UBE2J2/UBC6 Antibody
Immunogen
Produced in rabbits immunized with E. coli-derived Human UBE2J2/UBC6 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for UBE2J2/UBC6 Antibody
Western Blot: UBE2J2/UBC6 Antibody [NBP3-06347]
Western Blot: UBE2J2/UBC6 Antibody [NBP3-06347] - Anti-UBE2J1 rabbit polyclonal antibody at 1:2000 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:35 kDaObserved band size:40 kDa![Immunoprecipitation: UBE2J2/UBC6 Antibody [NBP3-06347]](https://resources.bio-techne.com/images/products/UBE2J2-UBC6-Antibody-Immunoprecipitation-NBP3-06347-img0002.jpg "Immunoprecipitation: UBE2J2/UBC6 Antibody [NBP3-06347]")
Immunoprecipitation: UBE2J2/UBC6 Antibody [NBP3-06347]
Immunoprecipitation: UBE2J2/UBC6 Antibody [NBP3-06347] - UBE2J1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-UBE2J1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody:Anti-UBE2J1 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 36 kDaObserved band size: 36 kDaApplications for UBE2J2/UBC6 Antibody
Application
Recommended Usage
Immunoprecipitation
1-5ul/mg of lysate
Western Blot
1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: UBE2J2
Long Name
Ubiquitin-conjugating Enzyme E2, J2
Alternate Names
NCUBE2, PRO2121, Ubc6, Ubc6p
Gene Symbol
UBE2J2
Additional UBE2J2 Products
Product Specific Notices for UBE2J2/UBC6 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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