UBE2M/Ubc12 Antibody (3C4)
Novus Biologicals, part of Bio-Techne | Catalog # H00009040-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3C4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
UBE2M (AAH58924, 1 a.a. ~ 183 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK
Specificity
UBE2M - ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for UBE2M/Ubc12 Antibody (3C4)
![Western Blot: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01]](https://resources.bio-techne.com/images/products/UBE2M-Ubc12-Antibody-3C4-Western-Blot-H00009040-M01-img0010.jpg "Western Blot: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01]")
Western Blot: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01]
Western Blot: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01] - UBE2M monoclonal antibody (M01), clone 3C4 Analysis of UBE2M expression in Jurkat.![Immunocytochemistry/ Immunofluorescence: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01]](https://resources.bio-techne.com/images/products/UBE2M-Ubc12-Antibody-3C4-Immunocytochemistry-Immunofluorescence-H00009040-M01-img0008.jpg "Immunocytochemistry/ Immunofluorescence: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01]")
Immunocytochemistry/ Immunofluorescence: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01]
Immunocytochemistry/Immunofluorescence: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01] - Analysis of monoclonal antibody to UBE2M on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01] - Detection limit for recombinant GST tagged UBE2M is approximately 0.1ng/ml as a capture antibody.
Applications for UBE2M/Ubc12 Antibody (3C4)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: UBE2M/Ubc12
Alternate Names
UBC-RS2, UBC12, UbcH12
Entrez Gene IDs
9040 (Human)
Gene Symbol
UBE2M
OMIM
603173 (Human)
UniProt
Additional UBE2M/Ubc12 Products
Product Documents for UBE2M/Ubc12 Antibody (3C4)
Product Specific Notices for UBE2M/Ubc12 Antibody (3C4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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