UBE2O Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03216

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all UBE2O Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3). LVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVAELSDSGQQEPEDGGPAPGEASQGSDSEGGAQGLASASRDHTDQTSETAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPECTEDK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

141 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE2O Antibody - BSA Free

Western Blot: UBE2O AntibodyBSA Free [NBP3-03216]

Western Blot: UBE2O AntibodyBSA Free [NBP3-03216]

Western Blot: UBE2O Antibody [NBP3-03216] - Analysis of extracts from normal (control) and UBE2O knockout (KO) HeLa cells, using UBE2O antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunocytochemistry/ Immunofluorescence: UBE2O Antibody - BSA Free [NBP3-03216]

Immunocytochemistry/ Immunofluorescence: UBE2O Antibody - BSA Free [NBP3-03216]

Immunocytochemistry/Immunofluorescence: UBE2O Antibody [NBP3-03216] - Analysis of HeLa cells using UBE2O antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: UBE2O AntibodyBSA Free [NBP3-03216]

Western Blot: UBE2O AntibodyBSA Free [NBP3-03216]

Western Blot: UBE2O Antibody [NBP3-03216] - Analysis of 200ug extracts of A-431 cells using UBE2O antibody . Western blot was performed from the immunoprecipitate using this antibody.
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Applications for UBE2O Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:4000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBE2O

UBE2O catalyzes the covalent attachment of ubiquitin to other proteins

Alternate Names

E2-230K, EC 6.3.2.19, FLJ12878, KIAA1734, Ubiquitin carrier protein O, ubiquitin-conjugating enzyme E2 O, Ubiquitin-conjugating enzyme E2 of 230 kDa, Ubiquitin-conjugating enzyme E2-230K, ubiquitin-conjugating enzyme E2O, Ubiquitin-protein ligase O

Gene Symbol

UBE2O

Additional UBE2O Products

Product Documents for UBE2O Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UBE2O Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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