UBE2R1/CDC34 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32153

Novus Biologicals, part of Bio-Techne
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NBP1-32153
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (97%), Rat (98%), Xenopus (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UBE2R1/CDC34 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human UBE2R1/CDC34. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (90%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE2R1/CDC34 Antibody

Immunocytochemistry/ Immunofluorescence: UBE2R1/CDC34 Antibody [NBP1-32153]

Immunocytochemistry/ Immunofluorescence: UBE2R1/CDC34 Antibody [NBP1-32153]

Immunocytochemistry/Immunofluorescence: UbcH3/Cdc34 Antibody [NBP1-32153] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDC34 protein stained by CDC34 antibody [N1C3] diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.
Immunocytochemistry/ Immunofluorescence: UBE2R1/CDC34 Antibody [NBP1-32153]

Immunocytochemistry/ Immunofluorescence: UBE2R1/CDC34 Antibody [NBP1-32153]

Immunocytochemistry/Immunofluorescence: UbcH3/Cdc34 Antibody [NBP1-32153] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.
UBE2R1/CDC34 Antibody UBE2R1/CDC34 Antibody

UbcH3/Cdc34 Antibody [NBP1-32153] -Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDC34 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

UbcH3/Cdc34 Antibody [NBP1-32153] -Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with CDC34 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for UBE2R1/CDC34 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UBE2R1/CDC34

Cdc34 is a ubiquitin conjugating enzyme (UBC) or E2 that has been shown to be essential for the G1 to S phase transition in S. cerevisiae. Human Cdc34 fully complements the budding yeast Cdc34 temperature sensitive mutant phenotype. In egg extracts of the frog Xenopus laevis, Cdc34 has been shown to be required for the onset of DNA replication and appears to function in a multi-protein complex. Also, Cdc34 is highly conserved among vertebrates and is likely to function in a similar manner in all eukaryotes to regulate the start of DNA synthesis.

Alternate Names

Cdc34, UBC3

Gene Symbol

CDC34

Additional UBE2R1/CDC34 Products

Product Documents for UBE2R1/CDC34 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for UBE2R1/CDC34 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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