UBE2T Antibody (1E12-4A3)

Novus Biologicals, part of Bio-Techne | Catalog # H00029089-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1E12-4A3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UBE2T Antibodies »

Product Specifications

Immunogen

UBE2T (AAH04152, 1 a.a. ~ 197 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MQRASRLKRELHMLATEPPPGITCWQDKDQMDDLRAQILGGANTPYEKGVFKLEVIIPERYPFEPPQIRFLTPIYHPNIDSAGRICLDVLKLPPKGAWRPSLNIATVLTSIQLLMSEPNPDDPLMADISSEFKYNKPAFLKNARQWTEKHARQKQKADEEEMLDNLPEAGDSRVHNSTQKRKASQLVGIEKKFHPDV

Specificity

UBE2T - ubiquitin-conjugating enzyme E2T (putative)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for UBE2T Antibody (1E12-4A3)

Western Blot: UBE2T Antibody (1E12-4A3) [H00029089-M01]

Western Blot: UBE2T Antibody (1E12-4A3) [H00029089-M01]

Western Blot: UBE2T Antibody (1E12-4A3) [H00029089-M01] - UBE2T monoclonal antibody (M01), clone 1E12-4A3 Analysis of UBE2T expression in Hela.
Immunoprecipitation: UBE2T Antibody (1E12-4A3) [H00029089-M01]

Immunoprecipitation: UBE2T Antibody (1E12-4A3) [H00029089-M01]

Immunoprecipitation: UBE2T Antibody (1E12-4A3) [H00029089-M01] - Analysis of UBE2T transfected lysate using anti-UBE2T monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with UBE2T MaxPab rabbit polyclonal antibody.

Applications for UBE2T Antibody (1E12-4A3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UBE2T

The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]).[supplied by OMIM]

Long Name

Ubiquitin-conjugating Enzyme E2T

Alternate Names

HSPC150, PIG50

Entrez Gene IDs

29089 (Human)

Gene Symbol

UBE2T

UniProt

AAH04152 (Human)

Additional UBE2T Products

Product Documents for UBE2T Antibody (1E12-4A3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for UBE2T Antibody (1E12-4A3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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