UBE2T Antibody (JE65-79)

Western Blot: UBE2T Antibody (JE65-79) [NBP3-33076]

Western Blot: UBE2T Antibody (JE65-79) [NBP3-33076] - Western blot analysis of UBE2T on different lysates with Rabbit anti-UBE2T antibody (NBP3-33076) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (15 ug/Lane)
Lane 2: K-562 cell lysate (15 ug/Lane)
Lane 3: MCF7 cell lysate (15 ug/Lane)
Lane 4: A549 cell lysate (15 ug/Lane)
Lane 5: HEK-293 cell lysate (15 ug/Lane)
Lane 6: Jurkat cell lysate (15 ug/Lane)
Lane 7: SKOV-3 cell lysate (15 ug/Lane)
Lane 8: SH-SY5Y cell lysate (15 ug/Lane)
Lane 9: HepG2 cell lysate (15 ug/Lane)
Lane 10: Mouse testis tissue lysate (30 ug/Lane)
Lane 11: Rat testis tissue lysate (30 ug/Lane)

Predicted band size: 22.5 kDa
Observed band size: 22.5 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33076) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: UBE2T Antibody (JE65-79) [NBP3-33076]

Immunocytochemistry/ Immunofluorescence: UBE2T Antibody (JE65-79) [NBP3-33076] - Immunocytochemistry analysis of HeLa cells labeling UBE2T with Rabbit anti-UBE2T antibody (NBP3-33076) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-UBE2T antibody (NBP3-33076) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.