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UBE2W Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92558

Novus Biologicals, part of Bio-Techne
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NBP1-92558

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DPPPGMTLNEKSVQNSITQWIVDMEGAPGTLYEGEKFQLLFKFSSRYPFDSPQVMFTGENIPVHPHVYSNGHICLSILTEDWSPALSVQSVCLSIISMLSSCKEKRRPPDNSFYVRTCNKNPKKTKWWY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UBE2W Antibody

Western Blot: UBE2W Antibody [NBP1-92558]

Western Blot: UBE2W Antibody [NBP1-92558]

Western Blot: UBE2W Antibody [NBP1-92558] - Analysis in control (vector only transfected HEK293T lysate) and UBE2W over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: UBE2W Antibody [NBP1-92558]

Immunohistochemistry-Paraffin: UBE2W Antibody [NBP1-92558]

Immunohistochemistry-Paraffin: UBE2W Antibody [NBP1-92558] - Staining of human Testis shows strong nuclear positivity in cells in seminiferous ducts and Leydig cells.

Applications for UBE2W Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Simple Western reported by internal validation: Tested in HeLa; separated by size.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBE2W

UBE2W catalyzes the covalent attachment of ubiquitin to other proteins

Long Name

Ubiquitin-conjugating Enzyme E2W

Alternate Names

Ubc16

Gene Symbol

UBE2W

Additional UBE2W Products

Product Documents for UBE2W Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UBE2W Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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