UBE3C Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20790

Novus Biologicals, part of Bio-Techne
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NBP2-20790
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UBE3C Antibodies »

Product Summary for UBE3C Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human UBE3C. The exact sequence is proprietary.

Reactivity Notes

Chicken (87%), Bovine (89%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

124 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE3C Antibody

Western Blot: UBE3C Antibody [NBP2-20790]

Western Blot: UBE3C Antibody [NBP2-20790]

Western Blot: UBE3C Antibody [NBP2-20790] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 5% SDS PAGE gel, diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: UBE3C Antibody [NBP2-20790]

Immunocytochemistry/ Immunofluorescence: UBE3C Antibody [NBP2-20790]

Immunocytochemistry/Immunofluorescence: UBE3C Antibody [NBP2-20790] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Western Blot: UBE3C Antibody [NBP2-20790]

Western Blot: UBE3C Antibody [NBP2-20790]

Western Blot: UBE3C Antibody [NBP2-20790] - Sample (30 ug of whole cell lysate) A: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.

Applications for UBE3C Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UBE3C

E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.

Alternate Names

EC 6.3.2.-, KIAA0010KIAA10ubiquitin-protein isopeptide ligase (E3), ubiquitin protein ligase E3C, ubiquitin-protein ligase E3C

Gene Symbol

UBE3C

UniProt

Q15386 (Human)

Additional UBE3C Products

Product Documents for UBE3C Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for UBE3C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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