UBE4A Antibody (2A5N2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15300

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15300-100ul
NBP3-15300-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2A5N2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all UBE4A Antibodies »

Product Summary for UBE4A Antibody (2A5N2)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE4A (Q14139). MTDQENNNNISSNPFAALFGSLADAKQFAAIQKEQLKQQSDELPASPDDSDNSVSESLDEFDYSVAEISRSFRSQQEICEQLNINHMIQRIFLITLDNSD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UBE4A Antibody (2A5N2)

Western Blot: UBE4A Antibody (2A5N2) [NBP3-15300]

Western Blot: UBE4A Antibody (2A5N2) [NBP3-15300]

Western Blot: UBE4A Antibody (2A5N2) [NBP3-15300] - Western blot analysis of extracts of HUVEC cells, using UBE4A antibody (NBP3-15300) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western Blot: UBE4A Antibody (2A5N2) [NBP3-15300]

Western Blot: UBE4A Antibody (2A5N2) [NBP3-15300]

Western Blot: UBE4A Antibody (2A5N2) [NBP3-15300] - Western blot analysis of extracts of various cell lines, using UBE4A antibody (NBP3-15300) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Applications for UBE4A Antibody (2A5N2)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBE4A

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly.

Alternate Names

E4MGC133315, KIAA0126ubiquitination factor E4A (homologous to yeast UFD2), ubiquitination factor E4A (UFD2 homolog, yeast), UBOX2, UFD2ubiquitin conjugation factor E4 A

Gene Symbol

UBE4A

Additional UBE4A Products

Product Documents for UBE4A Antibody (2A5N2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UBE4A Antibody (2A5N2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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