UBE4B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-58802
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Summary for UBE4B Antibody
Immunogen
The immunogen recognized by this antibody maps to a region between residue 125 and 175 of human ubiquitination factor E4B using the numbering given in entry AAD02233.1 (GeneID 10277).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for UBE4B Antibody
![Western Blot: UBE4B Antibody [NB100-58802]](https://resources.bio-techne.com/images/products/UBE4B-Antibody-Western-Blot-NB100-58802-img0003.jpg "Western Blot: UBE4B Antibody [NB100-58802]")
Western Blot: UBE4B Antibody [NB100-58802]
Western Blot: UBE4B Antibody [NB100-58802] - Detection of mouse UBE4B by western blot. Samples: Nuclear extract (50 ug) from NIH 3T3, TCMK-1, and Renca cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBE4B antibody NB100-58802 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.![Western Blot: UBE4B Antibody [NB100-58802]](https://resources.bio-techne.com/images/products/UBE4B-Antibody-Western-Blot-NB100-58802-img0002.jpg "Western Blot: UBE4B Antibody [NB100-58802]")
Western Blot: UBE4B Antibody [NB100-58802]
Western Blot: UBE4B Antibody [NB100-58802] - Detection of Human UBE4B by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBE4B antibody NB100-58802 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunoprecipitation: UBE4B Antibody [NB100-58802]](https://resources.bio-techne.com/images/products/UBE4B-Antibody-Immunoprecipitation-NB100-58802-img0004.jpg "Immunoprecipitation: UBE4B Antibody [NB100-58802]")
Immunoprecipitation: UBE4B Antibody [NB100-58802]
Immunoprecipitation: UBE4B Antibody [NB100-58802] - Detection of human UBE4B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UBE4B antibody NB100-58802 used for IP at 6 ug per reaction. UBE4B was also immunoprecipitated by rabbit anti-UBE4B antibody NB100-58803. For blotting immunoprecipitated UBE4B, NB100-58802 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for UBE4B Antibody
Application
Recommended Usage
Immunoprecipitation
1:10-1:500
Western Blot
1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: UBE4B
Long Name
Ubiquitination Factor E4B
Alternate Names
UBOX3, UFD2, UFD2a
Gene Symbol
UBE4B
UniProt
Additional UBE4B Products
Product Documents for UBE4B Antibody
Product Specific Notices for UBE4B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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