UBE4B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32737

Novus Biologicals, part of Bio-Techne
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NBP1-32737
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UBE4B Antibodies »

Product Summary for UBE4B Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human UBE4B. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

146 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE4B Antibody

Western Blot: UBE4B Antibody [NBP1-32737]

Western Blot: UBE4B Antibody [NBP1-32737]

Western Blot: UBE4B Antibody [NBP1-32737] - (30 ug of whole cell lysate) A: Hep G2 5% SDS PAGE diluted at 1:10000
Immunocytochemistry/ Immunofluorescence: UBE4B Antibody [NBP1-32737]

Immunocytochemistry/ Immunofluorescence: UBE4B Antibody [NBP1-32737]

Immunocytochemistry/Immunofluorescence: UBE4B Antibody [NBP1-32737] - Analysis of methanol-fixed Hep3B, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: UBE4B Antibody [NBP1-32737]

Immunohistochemistry-Paraffin: UBE4B Antibody [NBP1-32737]

Immunohistochemistry-Paraffin: UBE4B Antibody [NBP1-32737] - CA922 xenograft, using UBE4B antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for UBE4B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UBE4B

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Long Name

Ubiquitination Factor E4B

Alternate Names

UBOX3, UFD2, UFD2a

Gene Symbol

UBE4B

Additional UBE4B Products

Product Documents for UBE4B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for UBE4B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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