Ubiquitin-activating Enzyme/UBE1 Antibody (05)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05789
![Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (05) [NBP3-05789]](https://resources.bio-techne.com/images/products/Ubiquitin-activating-Enzyme-UBE1-Antibody-05-Western-Blot-NBP3-05789-img0001.jpg "Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (05) [NBP3-05789]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 05
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Ubiquitin-activating Enzyme/UBE1 (Accession#: NP_003325.2; Ser2-Arg1058).
Reactivity Notes
No cross-reactivity in ELISA with:
Insect cell lysate
Insect cell lysate
Specificity
No cross-reactivity in ELISA with: Insect cell lysate
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Ubiquitin-activating Enzyme/UBE1 Antibody (05)
Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (05) [NBP3-05789]
Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (05) [NBP3-05789] - Lane A: HepG2 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:111 kDaObserved band size:100 kDa![Immunoprecipitation: Ubiquitin-activating Enzyme/UBE1 Antibody (05) [NBP3-05789]](https://resources.bio-techne.com/images/products/Ubiquitin-activating-Enzyme-UBE1-Antibody-05-Immunoprecipitation-NBP3-05789-img0002.jpg "Immunoprecipitation: Ubiquitin-activating Enzyme/UBE1 Antibody (05) [NBP3-05789]")
Immunoprecipitation: Ubiquitin-activating Enzyme/UBE1 Antibody (05) [NBP3-05789]
Immunoprecipitation: Ubiquitin-activating Enzyme/UBE1 Antibody (05) [NBP3-05789] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg K562 Whole Cell Lysate0.5 uL anti-UBA1 mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-UBA1 mouse monoclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 111 kDaObserved band size: 111 kDaApplications for Ubiquitin-activating Enzyme/UBE1 Antibody (05)
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunoprecipitation
0.2-1 ul/mg of lysate
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ubiquitin-activating Enzyme/UBE1
Alternate Names
A1S9T, AMCX1, GXP1, POC20, SMAX2, UBA1, UBE1, Ubiquitinactivating Enzyme
Gene Symbol
UBA1
Additional Ubiquitin-activating Enzyme/UBE1 Products
Product Documents for Ubiquitin-activating Enzyme/UBE1 Antibody (05)
Product Specific Notices for Ubiquitin-activating Enzyme/UBE1 Antibody (05)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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