Ubiquitin-activating Enzyme/UBE1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76500

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Ubiquitin-activating Enzyme/UBE1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ubiquitin-activating Enzyme/UBE1 Antibody

Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NBP2-76500]

Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NBP2-76500]

Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody [NBP2-76500] - Analysis using Anti-UBA1 antibody NBP2-76500 (A) shows similar pattern to independent antibody NBP1-90307 (B).
Immunocytochemistry/ Immunofluorescence: Ubiquitin-activating Enzyme/UBE1 Antibody [NBP2-76500]

Immunocytochemistry/ Immunofluorescence: Ubiquitin-activating Enzyme/UBE1 Antibody [NBP2-76500]

Immunocytochemistry/Immunofluorescence: Ubiquitin-activating Enzyme/UBE1 Antibody [NBP2-76500] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.

Applications for Ubiquitin-activating Enzyme/UBE1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, containing 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ubiquitin-activating Enzyme/UBE1

E1 Ubiquitin Activating Enzyme is encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.

Alternate Names

A1S9T, AMCX1, GXP1, POC20, SMAX2, UBA1, UBE1, Ubiquitinactivating Enzyme

Gene Symbol

UBA1

Additional Ubiquitin-activating Enzyme/UBE1 Products

Product Documents for Ubiquitin-activating Enzyme/UBE1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ubiquitin-activating Enzyme/UBE1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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