UBL5 Antibody

Western Blot: UBL5 Antibody [NB600-470]

Western Blot: UBL5 Antibody [NB600-470] - Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of anti-GFP followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX. Panel C shows specific reaction with Hub1 using a 1:500 dilution of IgG fraction of Rabbit-anti-Hub1 (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX. All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4 C. Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-Hub1 is highly specific and does not cross react with other UBLs.

Western Blot: UBL5 Antibody [NB600-470]

Western Blot: UBL5 Antibody [NB600-470] - Anti-Hub1 raised from recombinant yeast Hub1. All UBLs possess limited homology to Ubiquitin & to each other; hence it is important to know degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins after reaction with 1:1,000 dilution of anti-GFP followed by reaction with 1:15,000 dilution of HRP Donkey-a-Goat IgG MX. Panel C shows specific reaction with Hub1 using 1:500 dilution of Rabbit-anti-Hub1 (Yeast) followed by reaction with 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX. All primaries were diluted in TTBS buffer with 5% non-fat milk & incubated with membranes overnight at 4C. Yeast lysate proteins separated by SDS-PAGE using a 15% gel. Data indicates that anti-Hub1 is highly specific & does not cross react with other UBLs. A chemiluminescence system was used for signal detection (Roche).