UBQLN4/CIP75 Antibody (BLR145J)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14725
Recombinant Monoclonal
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit Clone # BLR145J
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
residues 2-601 (full length)
Clonality
Monoclonal
Host
Rabbit
Scientific Data Images for UBQLN4/CIP75 Antibody (BLR145J)
![Immunoprecipitation: UBQLN4/CIP75 Antibody (BLR145J) [NBP3-14725]](https://resources.bio-techne.com/images/products/UBQLN4-CIP75-Antibody-Immunoprecipitation-NBP3-14725-img0001.jpg "Immunoprecipitation: UBQLN4/CIP75 Antibody (BLR145J) [NBP3-14725]")
Immunoprecipitation: UBQLN4/CIP75 Antibody (BLR145J) [NBP3-14725]
Immunoprecipitation: UBQLN4/CIP75 Antibody [NBP3-14725] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HEK293T cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiUBQLN4 recombinant monoclonal antibodyused for IP at 20 ul/mg lysate. UBQLN4was also immunoprecipitated by others rabbit anti-UBQLN4antibodies. For blottingimmunoprecipitated was used at1:1000. Chemiluminescence with an exposure time of 10seconds.
Western Blot: UBQLN4/CIP75 Antibody [NBP3-14725] -
Western Blot: UBQLN4/CIP75 Antibody [NBP3-14725] -Whole cell lysate (50 ug) from HEK293T, OVCAR-3, MCF-7, and K-562 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-UBQLN4 recombinant monoclonal antibody [BLR145J] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-COPB2 antibody.
Western Blot- UBQLN4/CIP75 Antibody [NBP3-14725] -
Western Blot- UBQLN4/CIP75 Antibody [NBP3-14725] -Whole cell lysate (50 ug) prepared using NETN buffer from MDA-MB-231 cells (WT and KO). Antibodies: Rabbit antiUBQLN4 recombinant monoclonal antibody [BLR145J] used for WB at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.Applications for UBQLN4/CIP75 Antibody (BLR145J)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100- 1:500
Immunohistochemistry
1:100 - 1:500
Immunohistochemistry-Paraffin
1:100 - 1:500
Immunoprecipitation
20 ul/1 mg lysate
Western Blot
1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Formulation, Preparation, and Storage
Purification
>95%
Formulation
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: UBQLN4/CIP75
Alternate Names
A1Uataxin-1 ubiquitin-like interacting protein, Ataxin-1 ubiquitin-like-interacting protein A1U, C1orf6ubiquilin-4, chromosome 1 open reading frame 6, UBINCIP75, ubiquilin 4
Gene Symbol
UBQLN4
Additional UBQLN4/CIP75 Products
Product Documents for UBQLN4/CIP75 Antibody (BLR145J)
Product Specific Notices for UBQLN4/CIP75 Antibody (BLR145J)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...