UBR2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45243
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Cited:
Block/Neutralize, Chemotaxis, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence SCPKSGGDKSRAPT corresponding to internal region according to NP_056070.1.
Epitope
SCPKSGGDKSRAPT
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 28740232). Mouse reactivity reported in scientific literature (PMID: 27897407).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for UBR2 Antibody
![Western Blot: UBR2 Antibody [NBP1-45243]](https://resources.bio-techne.com/images/products/UBR2-Antibody-Western-Blot-NBP1-45243-img0002.jpg "Western Blot: UBR2 Antibody [NBP1-45243]")
Western Blot: UBR2 Antibody [NBP1-45243]
Western Blot: UBR2 Antibody [NBP1-45243] - CD9-positive EVs induce catabolic response in C2C12 myotubes. EVs isolated from LCM were subjected to immunoprecipitation (IP) against CD9 with pre-immune IgG as control. Resulted pellet and supernatant (Sup) were used to treat C2C12 myotubes and compared with PBS and input EVs for catabolic activity using western blotting. Data (n=3) were analyzed by analysis of variance (ANOVA) and * denotes a difference from PBS treatment (P<0.05). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-017-00726-x), licensed under a CC-BY license.![Immunohistochemistry-Paraffin: UBR2 Antibody [NBP1-45243]](https://resources.bio-techne.com/images/products/UBR2-Antibody-Immunohistochemistry-Paraffin-NBP1-45243-img0001.jpg "Immunohistochemistry-Paraffin: UBR2 Antibody [NBP1-45243]")
Immunohistochemistry-Paraffin: UBR2 Antibody [NBP1-45243]
Immunohistochemistry-Paraffin: UBR2 Antibody [NBP1-45243] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
UBR2 Antibody [NBP1-45243] - Hsp70 and 90-deficient LLC tumor does not induce muscle catabolism in mice. TA of mice derived from b was analyzed by western blotting for markers of catabolic pathways. Data (n=6) were analyzed by ANOVA. * denotes a difference (P<0.05). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-017-00726-x), licensed under a CC-BY license.
Applications for UBR2 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
5ug/ml
Peptide ELISA
Detection limit 1:32000
Western Blot
0.1 ug/ml
Application Notes
WB: Preliminary experiments gave bands at approx. 150 kDa band in rat testis lysates after 0.01 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 199 kDa band according to rat NP_001171542.1. The detected band was successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Reviewed Applications
Read 1 review rated 5 using NBP1-45243 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: UBR2
Alternate Names
bA49A4.1, C6orf133MGC71112, chromosome 6 open reading frame 133, dJ242G1.1, dJ392M17.3, DKFZp686C08114, E3 ubiquitin-protein ligase UBR2, EC 6.3.2.-, KIAA0349FLJ36357, N-recognin-2, RP3-392M17.3, ubiquitin protein ligase E3 component n-recognin 2, Ubiquitin-protein ligase E3-alpha-2, Ubiquitin-protein ligase E3-alpha-II
Gene Symbol
UBR2
UniProt
Additional UBR2 Products
Product Documents for UBR2 Antibody
Product Specific Notices for UBR2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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