UBTF Antibody (2D8)

Novus Biologicals, part of Bio-Techne | Catalog # H00007343-M04

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2D8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UBTF Antibodies »

Product Specifications

Immunogen

UBTF (NP_055048, 551 a.a. ~ 650 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPAATNSSKKMKFQGEPKKPPMNGYQKFSQELLSNGELNHLPLKERMVEIGSRWQRISQSQKEHYKKLAEEQQKQYKVHLDLWVKSLSPQDRAAYKEYIS

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

UBTF - upstream binding transcription factor, RNA polymerase I (2D8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for UBTF Antibody (2D8)

Western Blot: UBTF Antibody (2D8) [H00007343-M04]

Western Blot: UBTF Antibody (2D8) [H00007343-M04]

Western Blot: UBTF Antibody (2D8) [H00007343-M04] - Analysis of UBTF expression in HepG2 (Cat # L019V1).
Immunocytochemistry/ Immunofluorescence: UBTF Antibody (2D8) [H00007343-M04]

Immunocytochemistry/ Immunofluorescence: UBTF Antibody (2D8) [H00007343-M04]

Immunocytochemistry/Immunofluorescence: UBTF Antibody (2D8) [H00007343-M04] - Analysis of monoclonal antibody to UBTF on HepG2 cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: UBTF Antibody (2D8) [H00007343-M04]

Immunohistochemistry-Paraffin: UBTF Antibody (2D8) [H00007343-M04]

Immunohistochemistry-Paraffin: UBTF Antibody (2D8) [H00007343-M04] - Analysis of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml
View 3 more images

Applications for UBTF Antibody (2D8)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UBTF

Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).[supplied by OMIM]

Alternate Names

90-KDa Nucleolus Organizer Region Autoantigen, Autoantigen NOR-90, NOR-90, nucleolar transcription factor 1, UBF, UBF1, UBF-1, UBF2, UBFUBF-1, upstream binding transcription factor, RNA polymerase I, Upstream-binding factor 1

Entrez Gene IDs

7343 (Human)

Gene Symbol

UBTF

OMIM

600673 (Human)

UniProt

NP_055048 (Human)

Additional UBTF Products

Product Documents for UBTF Antibody (2D8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UBTF Antibody (2D8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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