UBTF Antibody (6B6)

Novus Biologicals, part of Bio-Techne | Catalog # H00007343-M01

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Porcine, Bovine, Monkey

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6B6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UBTF Antibodies »

Product Specifications

Immunogen

UBTF (NP_055048, 551 a.a. ~ 650 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPAATNSSKKMKFQGEPKKPPMNGYQKFSQELLSNGELNHLPLKERMVEIGSRWQRISQSQKEHYKKLAEEQQKQYKVHLDLWVKSLSPQDRAAYKEYIS

Localization

Nuclear

Specificity

UBTF - upstream binding transcription factor, RNA polymerase I

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for UBTF Antibody (6B6)

Western Blot: UBTF Antibody (6B6) [H00007343-M01]

Western Blot: UBTF Antibody (6B6) [H00007343-M01] - UBTF monoclonal antibody (M01), clone 6B6 Analysis of UBTF expression in Hela S3 NE.

Immunocytochemistry/ Immunofluorescence: UBTF Antibody (6B6) [H00007343-M01]

Immunocytochemistry/Immunofluorescence: UBTF Antibody (6B6) [H00007343-M01] - Analysis of monoclonal antibody to UBTF on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: UBTF Antibody (6B6) [H00007343-M01]

Immunohistochemistry-Paraffin: UBTF Antibody (6B6) [H00007343-M01] - Analysis of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.

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Applications for UBTF Antibody (6B6)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UBTF

Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).[supplied by OMIM]

Alternate Names

90-KDa Nucleolus Organizer Region Autoantigen, Autoantigen NOR-90, NOR-90, nucleolar transcription factor 1, UBF, UBF1, UBF-1, UBF2, UBFUBF-1, upstream binding transcription factor, RNA polymerase I, Upstream-binding factor 1

Entrez Gene IDs

7343 (Human)

Gene Symbol

UBTF

OMIM

600673 (Human)

UniProt

NP_055048 (Human)

Additional UBTF Products

Product Documents for UBTF Antibody (6B6)

Product Datasheets

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COA

SDS

Product Specific Notices for UBTF Antibody (6B6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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