UCH-L3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-55316
Key Product Details
Species Reactivity
Validated:
Human, Zebrafish
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.5 mg/ml
Product Summary for UCH-L3 Antibody
Immunogen
Synthetic peptides corresponding to UCHL3(ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)) The peptide sequence was selected from the N terminal of UCHL3.
Peptide sequence MEGQRWLPLEANPEVTNQFLKQLGLHPNWQFVDVYGMDPELLSMVPRPVC The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for UCH-L3 Antibody
![Western Blot: UCH-L3 Antibody [NBP1-55316]](https://resources.bio-techne.com/images/products/UCH-L3-Antibody-Western-Blot-NBP1-55316-img0001.jpg "Western Blot: UCH-L3 Antibody [NBP1-55316]")
Western Blot: UCH-L3 Antibody [NBP1-55316]
Western Blot: UCH-L3 Antibody [NBP1-55316] - MCF7 cell lysate, Antibody Titration: 0.2-1 ug/ml.![Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP1-55316]](https://resources.bio-techne.com/images/products/UCH-L3-Antibody-Immunohistochemistry-Paraffin-NBP1-55316-img0003.jpg "Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP1-55316]")
Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP1-55316]
Immunohistochemistry-Paraffin: UCH-L3 Antibody [NBP1-55316] - Human placenta tissue at an antibody concentration of 4-8ug/ml.![Western Blot: UCH-L3 Antibody [NBP1-55316]](https://resources.bio-techne.com/images/products/UCH-L3-Antibody-Western-Blot-NBP1-55316-img0004.jpg "Western Blot: UCH-L3 Antibody [NBP1-55316]")
Western Blot: UCH-L3 Antibody [NBP1-55316]
Western Blot: UCH-L3 Antibody [NBP1-55316] - Antibody Positive control: Lane 1 : 30ug Zebrafish skin lysate Lane 2: 30ug Zebrafish liver lysate Primary Antibody Dilution: 1 : 1000 Secondary Antibody: Anti-rabbit-HRP Secondry Antibody Dilution: 1 : 4000.Applications for UCH-L3 Antibody
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
4-8 ug/ml
Western Blot
1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: UCH-L3
Long Name
Ubiquitin C-terminal Hydrolase L3
Alternate Names
Ubiquitin Thioesterase L3, UCHL3
Gene Symbol
UCHL3
UniProt
Additional UCH-L3 Products
Product Documents for UCH-L3 Antibody
Product Specific Notices for UCH-L3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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