UCP4 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94703
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 201-300 of human UCP4 (NP_004268.3). NQPRDKQGRGLLYKSSTD
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for UCP4 Antibody - Azide and BSA Free
![Western Blot: UCP4 AntibodyAzide and BSA Free [NBP2-94703]](https://resources.bio-techne.com/images/products/UCP4-Antibody-Western-Blot-NBP2-94703-img0002.jpg "Western Blot: UCP4 AntibodyAzide and BSA Free [NBP2-94703]")
Western Blot: UCP4 AntibodyAzide and BSA Free [NBP2-94703]
Western Blot: UCP4 Antibody [NBP2-94703] - Analysis of extracts of various cell lines, using UCP4 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.![Immunocytochemistry/ Immunofluorescence: UCP4 Antibody - Azide and BSA Free [NBP2-94703]](https://resources.bio-techne.com/images/products/UCP4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94703-img0001.jpg "Immunocytochemistry/ Immunofluorescence: UCP4 Antibody - Azide and BSA Free [NBP2-94703]")
Immunocytochemistry/ Immunofluorescence: UCP4 Antibody - Azide and BSA Free [NBP2-94703]
Immunocytochemistry/Immunofluorescence: UCP4 Antibody [NBP2-94703] - Analysis of U-2 OS cells using UCP4 at dilution of 1:100. Blue: DAPI for nuclear staining.Applications for UCP4 Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: UCP4
Alternate Names
FLJ33552, mitochondrial uncoupling protein 4, Solute carrier family 25 member 27, solute carrier family 25, member 27, UCP 4, UCP4uncoupling protein 4
Gene Symbol
SLC25A27
Additional UCP4 Products
Product Documents for UCP4 Antibody - Azide and BSA Free
Product Specific Notices for UCP4 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov