UGP2 Antibody (3H3)

Novus Biologicals, part of Bio-Techne | Catalog # H00007360-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3H3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all UGP2 Antibodies »

Product Specifications

Immunogen

UGP2 (NP_001001521, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSQDGASQFQEVIRQELELSVKKELEKILTTASSHEFEHTKKDLDGFRKLFHRFLQEKGPSVDWGKIQRPPEDSIQPYEKIKARGLPDNI

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic

Specificity

UGP2 - UDP-glucose pyrophosphorylase 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for UGP2 Antibody (3H3)

Western Blot: UGP2 Antibody (3H3) [H00007360-M01]

Western Blot: UGP2 Antibody (3H3) [H00007360-M01]

Western Blot: UGP2 Antibody (3H3) [H00007360-M01] - UGP2 monoclonal antibody (M01), clone 3H3. Analysis of UGP2 expression in NIH/3T3.
Western Blot: UGP2 Antibody (3H3) [H00007360-M01]

Western Blot: UGP2 Antibody (3H3) [H00007360-M01]

Western Blot: UGP2 Antibody (3H3) [H00007360-M01] - Analysis of UGP2 over-expressed 293 cell line, cotransfected with UGP2 Validated Chimera RNAi ( Cat # H00007360-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with UGP2 monoclonal antibody (M01), clone 3H3 (Cat # H00007360-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: UGP2 Antibody (3H3) [H00007360-M01]

Western Blot: UGP2 Antibody (3H3) [H00007360-M01]

Western Blot: UGP2 Antibody (3H3) [H00007360-M01] - UGP2 monoclonal antibody (M01), clone 3H3 Analysis of UGP2 expression in HeLa.
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Applications for UGP2 Antibody (3H3)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UGP2

The enzyme encoded by this gene is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MgUTP and forms UDP-glucose and MgPPi. In liver and muscle tissue, UDP-glucose is a direct precursor of glycogen; in lactating mammary gland it is converted to UDP-galactose which is then converted to lactose. The eukaryotic enzyme has no significant sequence similarity to the prokaryotic enzyme. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

EC 2.7.7.9, pHC379, UDPG, UDP-glucose diphosphorylase, UDP-glucose pyrophosphorylase, UDP-glucose pyrophosphorylase 2, UDPGP, UDPGP2, UGP1, UGPase, UGPase 2, UGPP2, uridyl diphosphate glucose pyrophosphorylase 2, UTP-glucose-1-phosphate uridyltransferase, UTP--glucose-1-phosphate uridylyltransferase, UTP--glucose-1-phosphate uridylyltransferase 2

Entrez Gene IDs

7360 (Human)

Gene Symbol

UGP2

OMIM

191760 (Human)

UniProt

NP_001001521 (Human)

Additional UGP2 Products

Product Documents for UGP2 Antibody (3H3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for UGP2 Antibody (3H3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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