ULK3 Antibody (PSH01-09)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33083
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH01-09
Concentration
1 mg/ml
Product Summary for ULK3 Antibody (PSH01-09)
Immunogen
Recombinant protein within human ULK3 aa 1-472 / 472. (Uniprot: Q6PHR2)
Localization
Cytoplasm.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ULK3 Antibody (PSH01-09)
Western Blot: ULK3 Antibody (PSH01-09) [NBP3-33083]
Western Blot: ULK3 Antibody (PSH01-09) [NBP3-33083] - Western blot analysis of ULK3 on different lysates with Rabbit anti-ULK3 antibody (NBP3-33083) at 1/1,000 dilution.Lane 1: 293T cell lysate (20 ug/Lane)
Lane 2: HeLa cell lysate (20 ug/Lane)
Lane 3: Jurkat cell lysate (20 ug/Lane)
Lane 4: Human brain tissue lysate (40 ug/Lane)
Lane 5: Mouse brain tissue lysate (40 ug/Lane)
Lane 6: Rat brain tissue lysate (40 ug/Lane)
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33083) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry: ULK3 Antibody (PSH01-09) [NBP3-33083]
Immunohistochemistry: ULK3 Antibody (PSH01-09) [NBP3-33083] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ULK3 antibody (NBP3-33083) at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33083) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry: ULK3 Antibody (PSH01-09) [NBP3-33083]
Immunohistochemistry: ULK3 Antibody (PSH01-09) [NBP3-33083] - Immunofluorescence analysis of paraffin-embedded human testis tissue labeling ULK3 with Rabbit anti-ULK3 antibody (NBP3-33083) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33083, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Applications for ULK3 Antibody (PSH01-09)
Application
Recommended Usage
Flow Cytometry
1:500-1:1000
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ULK3
Alternate Names
DKFZp434C131, EC 2.7.11.1, FLJ90566, serine/threonine-protein kinase ULK3, Unc-51-like kinase 3, unc-51-like kinase 3 (C. elegans)
Gene Symbol
ULK3
Additional ULK3 Products
Product Documents for ULK3 Antibody (PSH01-09)
Product Specific Notices for ULK3 Antibody (PSH01-09)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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