UNC13D/Munc 13-4 Antibody

Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NB100-41385]

Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NB100-41385] - Staining of paraffin embedded Human Thymus. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Knockdown Validated: UNC13D/Munc 13-4 Antibody [NB100-41385]

Knockdown Validated: UNC13D/Munc 13-4 Antibody [NB100-41385] - A549 cells stably expressing control shRNA (Ctrl) or Munc13-4 shRNA were left untreated (Untr) or were treated with TGFbeta-1 for 24 h, and SDS-PAGE Western blotting for indicated proteins was conducted. Image collected and cropped by CiteAb from the following publication (jcb.org/lookup/doi/10.1083/jcb.201710132), licensed under a CC-BY license.

Knockdown Validated: UNC13D/Munc 13-4 Antibody [NB100-41385]

Knockdown Validated: UNC13D/Munc 13-4 Antibody [NB100-41385] - SDS-PAGE Western blot of indicated proteins in MDA-MB-231 cells after stable expression of shRNA for Munc13-4 or Rab27a or a scrambled control (Ctrl). Image collected and cropped by CiteAb from the following publication (jcb.org/lookup/doi/10.1083/jcb.201710132), licensed under a CC-BY license.

Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] -

Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] - Munc13-4 KD strongly impairs exosome release. (A) SDS-PAGE Western blot of indicated proteins in MDA-MB-231 cells after stable expression of shRNA for Munc13-4 or Rab27a or a scrambled control (Ctrl). (B) Culture medium from MDA-MB-231 cells either untreated or stimulated w/ 1.25 µM ionomycin for 30 min centrifuged at 1,000 g to remove cellular debris & 10,000 g to remove large extracellular vesicles. (C) The resulting 10,000-g supernatant filtered onto a nitrocellulose membrane & analyzed for CD63, CD9, ALIX, & GM130 content by antibody blotting. (D) Quantification of CD63, CD9, & ALIX blots in C are shown as exosome release as a percentage of total cellular material w/ mean values ± standard error (SE) for n ≥ 3. *, P < 0.05 for comparison w/ corresponding control samples. (E) Panc-1 or A549 cells left untreated or treated w/ TGF beta-1 for 24 h. Indicated proteins detected by SDS-PAGE Western blot. (F) Panc-1 cells left untreated or treated w/ TGF beta-1 for 24 h, & Munc13-4 levels determined by immunofluorescence. TGF beta-1–treated cells exhibited a mesenchymal morphology. Bars, 5 μm. (G) A549 cells stably expressing control shRNA (Ctrl) or Munc13-4 shRNA left untreated (Untr) or treated w/ TGF beta-1 for 24 h, & SDS-PAGE Western blotting for indicated proteins conducted. (H) Culture media supernatants (as in B) from A549 cells that either untreated or stimulated w/ 1.25 µM ionomycin for 30 min filtered onto nitrocellulose membrane & analyzed for CD63 & GM130. (I) Quantification of CD63+ exosome release shown as a percentage of total cellular material w/ mean values ± SE for n = 5. *, P < 0.05; **, P < 0.01 for comparison w/ corresponding control samples. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29930202), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] -

Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] - Munc13-4 KD strongly impairs exosome release. (A) SDS-PAGE Western blot of indicated proteins in MDA-MB-231 cells after stable expression of shRNA for Munc13-4 or Rab27a or a scrambled control (Ctrl). (B) Culture medium from MDA-MB-231 cells either untreated or stimulated w/ 1.25 µM ionomycin for 30 min centrifuged at 1,000 g to remove cellular debris & 10,000 g to remove large extracellular vesicles. (C) The resulting 10,000-g supernatant filtered onto a nitrocellulose membrane & analyzed for CD63, CD9, ALIX, & GM130 content by antibody blotting. (D) Quantification of CD63, CD9, & ALIX blots in C are shown as exosome release as a percentage of total cellular material w/ mean values ± standard error (SE) for n ≥ 3. *, P < 0.05 for comparison w/ corresponding control samples. (E) Panc-1 or A549 cells left untreated or treated w/ TGF beta-1 for 24 h. Indicated proteins detected by SDS-PAGE Western blot. (F) Panc-1 cells left untreated or treated w/ TGF beta-1 for 24 h, & Munc13-4 levels determined by immunofluorescence. TGF beta-1–treated cells exhibited a mesenchymal morphology. Bars, 5 μm. (G) A549 cells stably expressing control shRNA (Ctrl) or Munc13-4 shRNA left untreated (Untr) or treated w/ TGF beta-1 for 24 h, & SDS-PAGE Western blotting for indicated proteins conducted. (H) Culture media supernatants (as in B) from A549 cells that either untreated or stimulated w/ 1.25 µM ionomycin for 30 min filtered onto nitrocellulose membrane & analyzed for CD63 & GM130. (I) Quantification of CD63+ exosome release shown as a percentage of total cellular material w/ mean values ± SE for n = 5. *, P < 0.05; **, P < 0.01 for comparison w/ corresponding control samples. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29930202), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] -

Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] - Munc13-4 KD strongly impairs exosome release. (A) SDS-PAGE Western blot of indicated proteins in MDA-MB-231 cells after stable expression of shRNA for Munc13-4 or Rab27a or a scrambled control (Ctrl). (B) Culture medium from MDA-MB-231 cells either untreated or stimulated w/ 1.25 µM ionomycin for 30 min centrifuged at 1,000 g to remove cellular debris & 10,000 g to remove large extracellular vesicles. (C) The resulting 10,000-g supernatant filtered onto a nitrocellulose membrane & analyzed for CD63, CD9, ALIX, & GM130 content by antibody blotting. (D) Quantification of CD63, CD9, & ALIX blots in C are shown as exosome release as a percentage of total cellular material w/ mean values ± standard error (SE) for n ≥ 3. *, P < 0.05 for comparison w/ corresponding control samples. (E) Panc-1 or A549 cells left untreated or treated w/ TGF beta-1 for 24 h. Indicated proteins detected by SDS-PAGE Western blot. (F) Panc-1 cells left untreated or treated w/ TGF beta-1 for 24 h, & Munc13-4 levels determined by immunofluorescence. TGF beta-1–treated cells exhibited a mesenchymal morphology. Bars, 5 μm. (G) A549 cells stably expressing control shRNA (Ctrl) or Munc13-4 shRNA left untreated (Untr) or treated w/ TGF beta-1 for 24 h, & SDS-PAGE Western blotting for indicated proteins conducted. (H) Culture media supernatants (as in B) from A549 cells that either untreated or stimulated w/ 1.25 µM ionomycin for 30 min filtered onto nitrocellulose membrane & analyzed for CD63 & GM130. (I) Quantification of CD63+ exosome release shown as a percentage of total cellular material w/ mean values ± SE for n = 5. *, P < 0.05; **, P < 0.01 for comparison w/ corresponding control samples. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29930202), licensed under a CC-BY license. Not internally tested by Novus Biologicals.