uNOS Antibody (NOS-3F7-B11 B5)

Novus Biologicals, part of Bio-Techne | Catalog # NB120-2801

Novus Biologicals, part of Bio-Techne
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NB120-2801
NB120-2801-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # NOS-3F7-B11 B5

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all uNOS Antibodies »

Product Specifications

Immunogen

Purified bovine bNOS.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Detects brain Nitric Oxide Synthase (bNOS), inducible NOS (iNOS) and epithelial NOS (eNOS).

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for uNOS Antibody (NOS-3F7-B11 B5)

Western Blot: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801]

Western Blot: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801]

Western Blot: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801] - Analysis of 25 ug of mouse brain (lane 1) and C2C12 (lane 2) lysates.
Immunocytochemistry/ Immunofluorescence: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801]

Immunocytochemistry/ Immunofluorescence: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801]

Immunocytochemistry/Immunofluorescence: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a uNOS monoclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.
Immunocytochemistry/ Immunofluorescence: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801]

Immunocytochemistry/ Immunofluorescence: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801]

Immunocytochemistry/Immunofluorescence: uNOS Antibody (NOS-3F7-B11 B5) [NB120-2801] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a uNOS monoclonal antibody at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.
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Applications for uNOS Antibody (NOS-3F7-B11 B5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:100

Immunoprecipitation

1:10 - 1:500

Western Blot

1:50 - 1:500
Application Notes
WB: Detects an approx. 130 kDa band representing iNOS in samples first induced with interferon (IFN) and lipopolysaccarides (LPS), an approx. 155 kDa band in tissues expressing bNOS and an approx. 140 kDa band in tissues expressing eNOS.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: uNOS

Nitric Oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells including vasorelaxation, neurotransmission and cytotoxicity. NO is produced by a Nitric Oxide Synthase (NOS) family of enzymes which oxidizes the amino acid L-arginine to form L-citrulline and NO.Two constitutive forms, brain NOS (bNOS) & endothelial cell NOS (eNOS), and one inducible form (iNOS), have been cloned showing regions for binding of calmodulin, NADPH, FAD, and FMN. iNOS has been found in a variety of tissues including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. It is induced after exposure to cytokines such as interferon-* (IFN), tumor necrosis factor, interleukin-1, interleukin-2, and lipopolysaccarides (LPS). After cytokine induction, iNOS has a delayed response followed by high levels of NO produced over a long period of time. eNOS and bNOS share ~50% sequence homology, and their activity depends on binding to the calcium/calmodulin complex. Both constitutive isoforms respond immediately to increased levels of calcium to produce low levels of NO over a short period of time. bNOS has been found in neurons, peripheral nerve cells, macula densa & pancreatic islet cells. eNOS is capable of synthesizing NO in vascular endothelial cells where it appears to play an important role in the control of vasotension and platelet aggregation.

Alternate Names

bNOS, Constitutive NOS, IHPS 1, IHPS1, N NOS, NC NOS, Neuronal nitric oxide synthase, Neuronal NOS, Nitric oxide synthase 1, Nitric oxide synthase 1 neuronal, Nitric oxide synthase brain, nNOS, NOS, Nos 1, NOS type I, NOS1

Entrez Gene IDs

18125 (Mouse); 24598 (Rat)

Gene Symbol

NOS1

Additional uNOS Products

Product Documents for uNOS Antibody (NOS-3F7-B11 B5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for uNOS Antibody (NOS-3F7-B11 B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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