UQCRC2 Antibody (2F6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15182
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2F6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for UQCRC2 Antibody (2F6)
Immunogen
A synthesized peptide derived from human UQCRC2. UniProt ID (P22695).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for UQCRC2 Antibody (2F6)
Western Blot: UQCRC2 Antibody (2F6) [NBP3-15182]
Western Blot: UQCRC2 Antibody (2F6) [NBP3-15182] - Positive WB detected in:HepG2 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate. All lanes: UQCRC2 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 49 KDa. Observed band size: 49 KDa.Immunohistochemistry: UQCRC2 Antibody (2F6) [NBP3-15182]
Immunohistochemistry: UQCRC2 Antibody (2F6) [NBP3-15182] - IHC image of UQCRC2 antibody diluted at 1:100 and staining in paraffin-embedded human liver tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Immunohistochemistry: UQCRC2 Antibody (2F6) [NBP3-15182]
Immunohistochemistry: UQCRC2 Antibody (2F6) [NBP3-15182] - IHC image of UQCRC2 antibody diluted at 1:100 and staining in paraffin-embedded human heart tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Applications for UQCRC2 Antibody (2F6)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: UQCRC2
Alternate Names
Complex III subunit 2, Core protein II, cytochrome b-c1 complex subunit 2, mitochondrial, QCR2, ubiquinol-cytochrome c reductase core protein II, Ubiquinol-cytochrome-c reductase complex core protein 2, UQCR2
Gene Symbol
UQCRC2
Additional UQCRC2 Products
Product Documents for UQCRC2 Antibody (2F6)
Product Specific Notices for UQCRC2 Antibody (2F6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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