UQCRC2 Antibody (3E3O8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16390
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3E3O8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for UQCRC2 Antibody (3E3O8)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 250-350 of human UQCRC2 (P22695). KANYRGGEIREQNGDSLVHAAFVAESAVAGSAEANAFSVLQHVLGAGPHVKRGSNTTSHLHQAVAKATQQPFDVSAFNASYSDSGLFGIYTISQATAAGDV
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for UQCRC2 Antibody (3E3O8)
Western Blot: UQCRC2 Antibody (3E3O8) [NBP3-16390]
Western Blot: UQCRC2 Antibody (3E3O8) [NBP3-16390] - Western blot analysis of extracts of various cell lines, using UQCRC2 Rabbit mAb (NBP3-16390) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Immunohistochemistry-Paraffin: UQCRC2 Antibody (3E3O8) [NBP3-16390]
Immunohistochemistry-Paraffin: UQCRC2 Antibody (3E3O8) [NBP3-16390] - Immunohistochemistry of paraffin-embedded human colon using UQCRC2 Rabbit mAb (NBP3-16390) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Western Blot: UQCRC2 Antibody (3E3O8) [NBP3-16390]
Western Blot: UQCRC2 Antibody (3E3O8) [NBP3-16390] - Western blot analysis of extracts of Mouse heart, using UQCRC2 Rabbit mAb (NBP3-16390) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.Applications for UQCRC2 Antibody (3E3O8)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: UQCRC2
Alternate Names
Complex III subunit 2, Core protein II, cytochrome b-c1 complex subunit 2, mitochondrial, QCR2, ubiquinol-cytochrome c reductase core protein II, Ubiquinol-cytochrome-c reductase complex core protein 2, UQCR2
Gene Symbol
UQCRC2
Additional UQCRC2 Products
Product Documents for UQCRC2 Antibody (3E3O8)
Product Specific Notices for UQCRC2 Antibody (3E3O8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...