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UQCRC2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80862

Novus Biologicals, part of Bio-Techne
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NBP1-80862
NBP1-80862-25ul

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IKAGSRYEDFSNLGTTHLLRLTSSLTTKGASSFKITRGIEAVGGKLSVTATRENMAYTVECLRGDVDILMEFLLNVTTAPEFRRWEVADLQPQLKIDKAVAFQNPQTHVIENLHAAAYRNALANPLYCPDYRIGKVTSEELHYFVQN

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (85%). Reactivity reported in scientific literature (PMID: 24086592)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UQCRC2 Antibody

Western Blot: UQCRC2 Antibody [NBP1-80862]

Western Blot: UQCRC2 Antibody [NBP1-80862]

Western Blot: UQCRC2 Antibody [NBP1-80862] - Analysis using Anti-UQCRC2 antibody NBP1-80862 (A) shows similar pattern to independent antibody NBP1-80861 (B).
Immunocytochemistry/ Immunofluorescence: UQCRC2 Antibody [NBP1-80862]

Immunocytochemistry/ Immunofluorescence: UQCRC2 Antibody [NBP1-80862]

Immunocytochemistry/Immunofluorescence: UQCRC2 Antibody [NBP1-80862] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80862]

Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80862]

Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80862] - Staining of human liver.

Applications for UQCRC2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UQCRC2

This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex),which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex

Alternate Names

Complex III subunit 2, Core protein II, cytochrome b-c1 complex subunit 2, mitochondrial, QCR2, ubiquinol-cytochrome c reductase core protein II, Ubiquinol-cytochrome-c reductase complex core protein 2, UQCR2

Entrez Gene IDs

7385 (Human)

Gene Symbol

UQCRC2

UniProt

Additional UQCRC2 Products

Product Documents for UQCRC2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UQCRC2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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