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Urocortin 3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-93444

Novus Biologicals, part of Bio-Techne
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NB100-93444

Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-KPRQDTAKSPHRTK corresponding to internal region according to NP_444277.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Urocortin 3 Antibody

Western Blot: Urocortin 3 Antibody [NB100-93444]

Western Blot: Urocortin 3 Antibody [NB100-93444]

Western Blot: Urocortin 3 Antibody [NB100-93444] - HEK293 lysate (10ug protein in RIPA buffer) over expressing Human UCN3 with DYKDDDDK tag probed with NB100-93444 (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with NB100-93444 (1mg/ml) in Lane B.

Applications for Urocortin 3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 2 ug/ml
Application Notes
WB: In transfected HEK293 transiently expressing full-length human UCN3 (myc and DYKDDDDK tagged), a band of approx. 19 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Urocortin 3

This gene is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is structurally related to the corticotropin-releasing factor (CRF) gene and the encoded product is an endogenous ligand for CRF type 2 receptors. In the brain it may be responsible for the effects of stress on appetite. In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin II.

Alternate Names

MGC119002, SPCSCP, Stresscopin, Ucn III, UCNIII, urocortin 3 (stresscopin), Urocortin III, urocortin-3

Entrez Gene IDs

114131 (Human)

Gene Symbol

UCN3

UniProt

Additional Urocortin 3 Products

Product Documents for Urocortin 3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Urocortin 3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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