Urocortin Antibody (2D7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62804

Novus Biologicals, part of Bio-Techne
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NBP2-62804
NBP2-62804-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoassay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2D7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Urocortin Antibodies »

Product Summary for Urocortin Antibody (2D7)

Immunogen

IMAC-purified SUMO-eXact-preproUCN2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Liew, O et al, His6-SUMO-eXact tag for producing human prepro-Urocortin 2 in Escherichia coli for raising monoclonal antibodies. J Immunol Method 2014, 403:37

Scientific Data Images for Urocortin Antibody (2D7)

Western Blot: Urocortin Antibody (2D7) [NBP2-62804]

Western Blot: Urocortin Antibody (2D7) [NBP2-62804]

Western Blot: Urocortin Antibody (2D7) [NBP2-62804] - Protein samples were separated by 16% SDS-PAGE for an hour at 200 V and electroblotted onto Immun-Blot PVDF membrane at 100 mA for 1 h. Western blotting was performed using this antibody (1:1000 dilution) as primary antibody and goat anti mouse IgG conjugated to horse radish peroxidase (1:2000). The band sizes are 35 and 80 kDa.

Applications for Urocortin Antibody (2D7)

Application
Recommended Usage

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Urocortin

Urocortin (UCN, UCN1) is a member of the widely expressed corticotropin releasing factor (CRF) peptide family that includes UCN1-3 and CRF. UCN stimulates cAMP and MAPK pathways via either of two G-protein coupled receptors, CRFR1 and CRFR2, while other family members prefer CRFR1 (CRF) or CRFR2 (UCN2, UCN3). In the brain, UCN has been implicated in counteracting stress-provoked anxiety and suppressing appetite. It also modulates metabolic activity in peripheral tissues and protects the heart from ischemia/reprofusion injury. The UCN cDNA encodes 124 aa, including a 25 aa signal sequence, a 57 aa propeptide, and a 42 aa mature active 5 kDa UCN peptide. The proform of human UCN (aa 26-124, 11 kDa predicted) shares approximately 80% aa sequence identity with mouse and rat UCN.

Alternate Names

UCN, UI, UROC

Gene Symbol

UCN

Additional Urocortin Products

Product Documents for Urocortin Antibody (2D7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Urocortin Antibody (2D7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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