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UROD Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85946

Novus Biologicals, part of Bio-Techne
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NBP1-85946

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VPGKGPSFPEPLREEQALDRLRDPEVVASELGYVFQAITLTRQRLAGRVPLIGFAGAPWTLMTYMVEGGGSSTMAQAKRWLYQRPQASHQLLRILTD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UROD Antibody

Western Blot: UROD Antibody [NBP1-85946]

Western Blot: UROD Antibody [NBP1-85946]

Western Blot: UROD Antibody [NBP1-85946] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: UROD Antibody [NBP1-85946]

Immunohistochemistry-Paraffin: UROD Antibody [NBP1-85946]

Immunohistochemistry-Paraffin: UROD Antibody [NBP1-85946] - Staining of human small intestine shows strong cytoplasmic and membranous positivity in glandular cells.
Western Blot: UROD Antibody [NBP1-85946]

Western Blot: UROD Antibody [NBP1-85946]

Western Blot: UROD Antibody [NBP1-85946] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-621

Applications for UROD Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UROD

This gene encodes the fifth enzyme of the heme biosynthetic pathway. This enzyme is responsible for catalyzing the conversion of uroporphyrinogen to coproporphyrinogen through the removal of four carboxymethyl side chains. Mutations and deficiency in this enzyme are known to cause familial porphyria cutanea tarda and hepatoerythropoetic porphyria

Alternate Names

EC 4.1.1.37, PCT, UPD, URO-D, uroporphyrinogen decarboxylase, uroporphyrinogen III decarboxylase

Gene Symbol

UROD

Additional UROD Products

Product Documents for UROD Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UROD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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