Uromodulin Antibody (3F10)

Novus Biologicals, part of Bio-Techne | Catalog # H00007369-M03

Novus Biologicals, part of Bio-Techne
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H00007369-M03
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3F10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Uromodulin Antibodies »

Product Specifications

Immunogen

UMOD (NP_001008390.1, 25 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DTSEARWCSECHSNATCTEDEAVTTCTCQEGFTGDGLTCVDLDECAIPGAHNCSANSSCVNTPGSFSCVCPEGFRLSPGLGCTDVDECAEPGLSHC

Specificity

Reacts with uromodulin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Uromodulin Antibody (3F10)

Western Blot: Uromodulin Antibody (3F10) [H00007369-M03]

Western Blot: Uromodulin Antibody (3F10) [H00007369-M03]

Western Blot: Uromodulin Antibody (3F10) [H00007369-M03] - Analysis of UMOD expression in transfected 293T cell line by UMOD monoclonal antibody (M03), clone 3F10. Lane 1: UMOD transfected lysate (Predicted MW: 66.7 KDa). Lane 2: Non-transfected lysate.
Western Blot: Uromodulin Antibody (3F10) [H00007369-M03]

Western Blot: Uromodulin Antibody (3F10) [H00007369-M03]

Western Blot: Uromodulin Antibody (3F10) [H00007369-M03] - Analysis of UMOD transfected lysate using anti-UMOD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with UMOD rabbit polyclonal antibody.
ELISA: Uromodulin Antibody (3F10) [H00007369-M03]

ELISA: Uromodulin Antibody (3F10) [H00007369-M03]

ELISA: Uromodulin Antibody (3F10) [H00007369-M03] - Detection limit for recombinant GST tagged UMOD is 0.3 ng/ml as a capture antibody.

Applications for Uromodulin Antibody (3F10)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in WB and ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Uromodulin

This gene encodes uromodulin, the most abundant protein in normal urine. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. Uromodulin may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of uromodulin in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the autosomal dominant renal disorders medullary cystic kidney disease-2 (MCKD2) and familial juvenile hyperuricemic nephropathy (FJHN). These disorders are characterized by juvenile onset of hyperuricemia, gout, and progressive renal failure. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same isoform. [provided by RefSeq]

Alternate Names

ADMCKD2, FJHN, HNFJ, MCKD2, THGP, THP, UMOD, Uromucoid

Entrez Gene IDs

7369 (Human)

Gene Symbol

UMOD

UniProt

NP_001008390 (Human)

Additional Uromodulin Products

Product Documents for Uromodulin Antibody (3F10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Uromodulin Antibody (3F10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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