Uroplakin III Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29477

Novus Biologicals, part of Bio-Techne
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NBP2-29477
NBP2-29477-20ug
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NBP2-33126
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all Uroplakin III Antibodies »

Product Specifications

Immunogen

A synthetic peptide, aa260-279 (GASESSYTSVNRGPPLDRAE) of human Uroplakin III (Uniprot: O75631)

Reactivity Notes

The amino acid sequence used as the immunogen for this UPK3A antibody is 100% homologous in human and 85% in mouse and rat.

Localization

Cell surface

Marker

Bladder Carcinoma Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-33126)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Uroplakin III Antibody

Western Blot: Uroplakin III Antibody [NBP2-29477]

Western Blot: Uroplakin III Antibody [NBP2-29477]

Western Blot: Uroplakin III Antibody [NBP2-29477] - Human HEK293 lysate in the 1) absence and 2) presence of immunizing peptide using Uroplakin III antibody at 4 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Applications for Uroplakin III Antibody

Application
Recommended Usage

Western Blot

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Uroplakin III

UPK3A, is an abbreviation for Uroplakin-3A, also called UP3a and UPIIIa, is a component of the asymmetric unit membrane (AUM) that is a highly specialized biomembrane made by terminally differentiated urothelial cells. The protein may play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. UPK3A also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence through FimH bacterial protein binding leading to bladder infection. FimH initiates bladder pathology by binding to the UPK3A uroplakin receptor complex, but the subsequent events mediating pathogenesis have not been fully characterized. Bacteria-induced UPK3a signaling may mediate bladder responses to insult by uropathogenic E. coli or UPEC. Besides structure and function, UPK3A also may be a tumor cell marker inversely expressed with malignancy grade of bladder cancer and it has already been shown as a urinary marker for the detection of bladder cancer. Antibody to UPK3A is a useful tool for characterizing the anti-apoptotic, structural and functional activities of this molecule.

Alternate Names

MGC119178, UP3a, UPIII, UPIIIA, UPK3, UPK3A, uroplakin 3, uroplakin 3A, Uroplakin III, uroplakin3a, uroplakin-3a

Gene Symbol

UPK3A

UniProt

O75631 (Human)

Additional Uroplakin III Products

Product Documents for Uroplakin III Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Uroplakin III Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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