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USF2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12887

Novus Biologicals, part of Bio-Techne
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NBP3-12887

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human USF2. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for USF2 Antibody

Western Blot: USF2 Antibody [NBP3-12887]

Western Blot: USF2 Antibody [NBP3-12887]

Western Blot: USF2 Antibody [NBP3-12887] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with USF2 antibody (NBP3-12887) diluted at 1:500 and competitor's antibody diluted by 1:200.
Immunocytochemistry/ Immunofluorescence: USF2 Antibody [NBP3-12887]

Immunocytochemistry/ Immunofluorescence: USF2 Antibody [NBP3-12887]

Immunocytochemistry/Immunofluorescence: USF2 Antibody [NBP3-12887] - USF2 antibody detects USF2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: USF2 stained by USF2 antibody (NBP3-12887) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.
Western Blot: USF2 Antibody [NBP3-12887]

Western Blot: USF2 Antibody [NBP3-12887]

Western Blot: USF2 Antibody [NBP3-12887] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with USF2 antibody (NBP3-12887) diluted at 1:500.

Applications for USF2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: USF2

USF2 encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. Two transcript variants encoding distinct isoforms have been identified for this gene.

Alternate Names

BHLHB12, bHLHb12upstream stimulatory factor 2, Class B basic helix-loop-helix protein 12, FIPc-fos interacting protein, FOS-interacting protein, Major late transcription factor 2, Upstream transcription factor 2, upstream transcription factor 2, c-fos interacting

Gene Symbol

USF2

Additional USF2 Products

Product Documents for USF2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for USF2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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